General description

Recombinant, human apo-tissue factor expressed in S. frugiperda insect cells using a baculovirus expression system. Cell-surface protein of endothelial cells and monocytes involved in coagulation and inflammation. Primary cellular initiator of coagulation protease cascade. Functions as receptor and cofactor for coagulation Factors VII and VIIa. Requires lipidation for clotting assays or other functional activities.

M.W. 35,000-37,000.

Recombinant, human apo-tissue factor expressed in S. frugiperda insect cells using a baculovirus expression system. Cell-surface protein of endothelial cells and monocytes involved in coagulation and inflammation. Primary cellular initiator of coagulation protease cascade. Requires lipidation for clotting assays or other functional activities. Functions as receptor and cofactor for coagulation Factors VII and VIIa.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wildgoose, P., et al. 1993. Biochemistry 32, 114.Neuenschwander, PF., et al. 1993. J. Biol. Chem.268, 21489.Yamamoto, M., et al. 1992. J. Biol. Chem.267, 19089.Morrissey, J., et al. 1991. Thrombosis Res.52, 247.Drake, T.A., et al. 1989. J. Cell Biol.109, 389.Bach, R., et al. 1981. J. Biol. Chem.256, 8324.

Packaging

25 µg in Plastic ampoule

Physical form

Solid lyophilized from 200 mM mannitol, 150 mM NaCl, 10 mM Tris-HCl, 0.01% CHAPS, pH 8.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C).

Reconstitute with 1 ml distilled H₂O

Warning

Toxicity: Standard Handling (A)